Figure 6
From: Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
CD200R1 expression in BV2 cells: effect of C/EBPβ overexpression. (A) CD200R1 mRNA expression in control and LPS-treated (six hours) BV2-pCDNA and BV2-LAP cell clones. (B-D) CD200R1 protein in control and LPS-treated (24 hours) BV2-pCDNA and BV2-LAP cells according to flow cytometry. (B) Histogram plots, representative of three independent experiments, showing CD200R1 staining in the BV2 cell clones. (C) Quantification of CD200R1 positive cells and fluorescence intensity (D) in each experimental group. CD200R1 mRNA and protein expression was lower in BV2-LAP cells than in BV2-pCDNA cells, due to an increase in both the number of CD200R1 positive cells and the level of CD200R1 protein expression. Bars are means + SEM of three independent experiments. *P <0.05, **P <0.01 and ***P <0.001 versus C; ##P <0.01 and ###P <0.001 versus the equivalent experimental condition in BV2-pCDNA cells. Two-way ANOVA and Bonferroni post-test. ANOVA, analysis of variance; C, control; C/EBPβ, CCAAT/enhancer binding protein β; LPS, lipopolysaccharide; SEM, standard error of the mean.