Delayed inflammatory protein expression at 4 weeks and 4 months after contusive rat SCI. Protein expression of microglia/macrophages marker Iba-1 and NADPH oxidase component p22PHOX were analyzed using Western blotting at 4 weeks and 4 months post-injury. (A-B) Representative Western blots of Iba-1, p22PHOX and the loading control at 4 weeks (A) and 4 months (B) after SCI. (C) Western blot analysis of Iba-1 and p22PHOX protein expression indicated a significant increase at 4 weeks after SCI followed by a prolonged upregulation for up to 4 months post-injury. Bars represent mean ± SEM. *p < 0.05 compared with sham group. n = 4-6 rats per time point.