Figure 2

Neutrophil accumulation in the injured cortex 24 hours after injury is dramatically reduced in neutropenic mice. A) Micrographs of Gr-1 positive cells (green) 24 hours after CCI in the cortex and hippocampus. Blue represents nuclear staining using DAPI. Scalebar is 100 μm. B) Quantification of Gr-1 positive cells in the cortex and hippocampus of intact (black bars, n = 7) and PMN-depleted (open bars, n = 7) mice. * indicates significant difference at p < 0.05. Values are mean ± SEM.