Figure 3

Brain water content but not macromolecule extravasation following TBI is affected by neutrophil depletion. A) Brain water content in the contralateral and ipsilateral areas 24 and 48 hours after CCI in intact (black bars, 24 hrs: n = 8, 48 hrs: n = 9) and PMN-depleted (open bars, 24 hrs: n = 8, 48 hrs: n = 9) mice. Values for naïve mice are provided for comparison. B) Evan's blue content in the contralateral and ipsilateral sides 24 and 48 hours after CCI in intact (black bars, 24 hrs: n = 8, 48 hrs: n = 5) and PMN-depleted (open bars, 24 hrs: n = 5, 48 hrs: n = 6) mice. Dashed line represents baseline EB leakage in naïve mice 24 hours after injury for comparison. * indicates significant difference at p < 0.05. Values are mean ± SEM. C) Representative image of EB leakage 24 hours following CCI.