Table 1 Experimental set up.
Sample size | Time of sacrifice | Analysis | |||
|---|---|---|---|---|---|
Naïve | Naïve + PMN depletion | CCI | CCI + PMN depletion |  |  |
| Â | Â | 8 | 5 | 24 hours | Evan's blue extraction (EB injection given 4 hours after CCI) |
| Â | Â | 7 | 7 | 24 hours | Cleaved caspase-3 Neutrophil accumulation |
6 | 6 | 8 | 8 | 24 hours | Brain water content |
2 | 3 | 5 | 6 | 48 hours | Evan's blue extraction (EB injection given 24 hours after CCI) |
| Â | Â | 9 | 9 | 48 hours | Brain water content |
| Â | Â | 5 | 5 | 7 days | Microglial/macrophage activation Brain tissue loss |
| Â | Â | 5 | 5 | 14 days | Brain tissue loss |