Figure 1

Characterization of BMVECs by von Willebrand Factor (VWF) and vimentin. (A–B). BMVECs were stained for VWF: phase image + DAPI (blue) shown in panel A; VWF (green) + DAPI (blue) shown in panel B. (C–D) Identical BMVEC cultures were grown with the same secondary antibody, only lacking the VWF primary antibody: phase image + DAPI (blue) shown in panel C; no primary antibody + DAPI (blue) shown in panel D. (E–F) Phase images and fluorescent images of BMVECs stained for vimentin. (G) BMVECs lacking staining for the astrocyte marker GFAP. (H) Positive staining for GFAP on astrocyte cultures. Original microscope magnification = 600 × for panels A–D, 400 × for E and F, and 200 × for panels G and H.