Figure 4

Effects of sodium nitroprusside (SNP) on the release of sPLA ₂ -IIA from BMVECs. BMVECS were treated with 5 μg/ml of LPS for 16 h, and the culture medium was Western blotted with antibodies against sPLA₂-IIA. (A) Cells were pretreated with or without 1 μM of SNP for 15 min before LPS stimulus. The blots shown are representative of one of three separate experiments, the same membrane was re-probed for BSA, and the relative sPLA₂ band intensity was calculated as shown in the bar graph below the blot. (B) Corresponding nitrite levels for each condition by LPS treatment with SNP. The data shown are the averages of four separate experiments (means ± SE), *P < 0.05 versus their respective normal groups, ^{^} P < 0.05 versus their respective LPS alone groups.