signal contributes to the migration of microglia in BV-2 microglia cells. (A) P2X7-specific shRNA transfection significantly reduced the increase in FasL generation induced by the OCM from OGD-stimulated cortical neurons. **P <0.01 vs. Control; #
P <0.05, ##
P <0.01 vs. OCM treatment alone. (B) OCM-induced FasL protein expression was abolished by lentiviral infection of P2X7-shRNA in the BV-2 cell line. Quantified data are shown in (C). **P <0.01 vs. Control; #
P <0.05 vs. OCM treatment alone. (D) Representative immunoblots (upper) and quantitative analyses (lower) for FasL from BV-2 cultures following actinomycin D or anisomycin treatment. *P <0.05 vs. Control; #
P <0.05 vs. OCM treatment alone. OCM, OGD-treated cell-free conditioned medium.