Figure 6

FasL is required for microglial migration. (A) A transwell assay was used to examine whether FasL silence or lentiviral infection of P2X₇-shRNA could attenuate recombinant FasL-mediated microglial migration. **P <0.01 vs. Control; ^## P <0.01 vs. recombinant FasL treatment alone. (B) FasL antibody neutralization or the loss of P2X₇ receptors abrogates the response of microglia to ATP in primary microglial cell culture. Microglia from wild-type or P2X₇ ^−/− mice visualized using time-lapse microscopy. Exuberant process extension toward a source point of ATPγS (1 mM from injection needle, red) was observed in the microglia of wild-type but not P2X₇ ^−/− mice or preincubation with FasL antibody. Scale bar = 20 μm.