Figure 8
Microglia-induced neuronal injury in conjunction with P2X 7 /FasL pathway activation. (A) Neurological scores were determined 168 h after microsphere embolism in P2X7 −/− mice. The data are expressed as the mean ± SEM (n = 6 mice). *P <0.05 vs. WT mice. (B) Immunoblots demonstrating FasL/Fas cell death signaling measured 168 h after microsphere embolism in P2X7 −/− and WT mice. Quantified data are shown in (C). Data are expressed as means ± SEM (n = 6 mice). *P <0.05; **P <0.01 vs. Sham (WT); # P <0.05 vs. ME (WT) group. S-WT, Sham (wild type). (D) Decreased FasL immunostaining was observed in P2X7 −/− mice 168 h after ME. DAPI counterstaining indicates cell nuclei (blue). Scale bar = 100 μm. (E) Fluoro-Jade C staining demonstrating the degree of neurodegeneration in WT and gld/gld mice 168 h after ME. Scale bar = 50 μm. ME, microsphere embolism. (F). Immunohistochemical staining of FasL and ED1 in the ischemic brains of WT and gld/gld mice 168 h after ME. Scale bar = 50 μm.