Downstream Toll-like receptor signaling mediates adaptor-specific cytokine expression following focal cerebral ischemia

Table 2 TRIF-dependent cytokine/chemokines following permanent middle cerebral artery occlusion

Location		WT	MyD88^−/−	TRIF	P-value
Serum (pg/ml)
IP-10	Baseline	164.8 ± 25.1	124.1 ± 17.6	147.5 ± 35.9	n.s.
	3 hours	121.4 ± 11.4	105.9 ± 37.0	104.9 ± 18.9	n.s.
	24 hours	164.7 ± 9.1	135.8 ± 21.9	113.9 ± 16.7	<0.05^†
MIP-1α	Baseline	169.3 ± 28.1	140.9 ± 153.9	41.7 ± 72.2	<0.05^†
	3 hours	166.4 ± 23.4	71.5 ± 62.7	100.4 ± 68.3	n.s.
	24 hours	145.7 ± 22.6	6.7 ± 11.6	30.9 ± 53.5	<0.05^†
Brain (pg/ml/g)
IP-10	Baseline	1,095.9 ± 159.5	985.4 ± 207.7	619.2 ± 141.2	<0.05^†
	3 hours	2,077.2 ± 803.6	1087.5 ± 190.4	710.5 ± 223.0	0.039,^* <0.05^†
	24 hours	2,997.3 ± 2,074.2	5169.9 ± 3305	2,820 ± 1,273	n.s.
MIP-1α	Baseline	1,096.3 ± 372.6	1026.1 ± 434.6	228.2 ± 395.3	<0.05^†
	3 hours	1,267.8 ± 210.9	891.5 ± 501.6	171.2 ± 148.7	<0.05^†
	24 hours	1,088.2 ± 413.6	1293.9 ± 741.1	403.1 ± 355.7	n.s.

IP-10 inducible protein 10, MIP-1α macrophage inflammatory protein 1α, TRIF Toll/interleukin 1 receptor domain-containing adaptor-inducing interferon β, WT wild type.
^*Significantly different between all three groups using Kruskal-Wallis.
^†Significantly different between WT and TRIF mutant using Mann–Whitney test.
Parentheses: fold change compared to baseline for that particular group.

ISSN: 1742-2094