Figure 1From: JNK signaling is the shared pathway linking neuroinflammation, blood–brain barrier disruption, and oligodendroglial apoptosis in the white matter injury of the immature brain Upregulation of neuroinflammation, blood–brain barrier damage and cell apoptosis in association with white matter injury in P2 rat pups after lipopolysaccharide-sensitized hypoxic-ischemia. On P11 in the LPS + HI group, Nissl staining (A) showed no significant injury in the cortex (gross picture in the upper panel; microscopic picture in the lower panel photographed from the cortex marked with an asterisk). (B) Immunohistochemical staining demonstrated that the LPS + HI group had markedly decreased MBP expression and increased GFAP-positive astrogliosis in the white matter of the ipsilateral hemisphere compared to the control and NS + HI groups. (C) Immunohistochemistry 24 h post-insult showed that the LPS + HI but not the NS + HI group had significant increases in ED1-positive microglia, TNF-α immunoreactivities, IgG extravasation, and cleaved caspase 3-positive apoptotic cells in the white matter. Microscopic pictures of (B,C) were taken from the white matter area marked with a circle in (A). ED1, microglia marker; GFAP, glial fibrillary acidic protein; HI, hypoxic-ischemia; LPS, lipopolysaccharide; MBP, myelin basic protein; NS, normal saline; P, postpartum. Scale bar = 200 μm for MBP, 50 μm for cleaved caspase 3, and 100 μm for the others.Back to article page