Figure 5

Protein analysis of M1 versus M2 markers after PNS injury. Western blot analysis showing the arginase-1 protein induction in axotomized sciatic nerves. The IL-4/IL-13 stimulated peritoneal macrophages were used as a positive control (M2) (a). Western blot analysis showing iNOS protein induction in axotomized sciatic nerves. Peritoneal macrophages stimulated with IFNγ/LPS served as a positive control (M1) (b). For western blot analysis, MW values expressed in kDa are shown at the right side of the blots. Immunohistochemical staining of sciatic nerve slices for arginase-1 and iNOS at different time points after injury (c). Blood vessels in the nerves stain positive for iNOS by immunohistochemical staining (d). Scale bar 50 μm.