Figure 1

IL-1β regulates expression of 29 of 92 human inflammation genes; C/EBPβ knockdown affects 17 of the 29 genes. Primary human astrocytes, from two independent donors, were transfected with siCON or siC/EBPβ, cultured for 24 h and then treated with IL-1β (20 ng/ml) for 12 h. Total RNA was isolated, reverse-transcribed and then assayed for human inflammation gene expression using the TaqMan® Human Inflammation Assay. Data are illustrated as a heat map; red and green represent positive and negative fold-change, respectively. Increasing color contrast corresponds to increasing absolute fold-change. Orange and blue represent positive and negative percent change, respectively, in target mRNA levels in Emphasis>/Emphasis>/EBPβ + IL-1β versus IL-1β. Each individual TaqMan® assay of the Human Inflammation Array was independently analyzed by one-way ANOVA followed by Newman-Keuls post-test to compare among control, IL-1β and Emphasis>/Emphasis>/EBPβ treatments. Significance was set at p < 0.05. (Newman-Keuls post-test: control versus IL-1β, * = p < 0.05; control versus siC/EBPβ + IL-1β, ^ = p < 0.05; siC/EBPβ + IL-1β versus IL-1β, # = p < 0.05).