Figure 5
From: C/EBPβ regulates multiple IL-1β-induced human astrocyte inflammatory genes
IL-1β signals through p38K to increase astrocyte COX-2 protein. (A-B) Astrocytes were cultured for 24 h, pretreated with pathway-selective inhibitors (SB203580-20 μM and U0126-20 μM) and then treated with IL-1β for 24 h. Total protein lysates were isolated, resolved by SDS-PAGE, transferred to a PVDF membrane and then immunoblotted for COX-2 expression. (B) COX-2 and β-actin bands were analyzed by densitometry analysis, and their intensities graphed using β-actin as the loading control; p-values are compared to control unless denoted by lines to specific comparisons. Densitometry fold change data are cumulative from two independent astrocyte donors (n = 2).