Figure 5From: CXCL1 can be regulated by IL-6 and promotes granulocyte adhesion to brain capillaries during bacterial toxin exposure and encephalomyelitis Contribution of CXCL1 to the development of EAE. a, Clinical scores of EAE mice injected intravenously once daily from day 7 with 4 mg/kg anti-CXCL1 antibody or isotypic control antibody. The means were compared by multivariate ANOVA with repeated measures (P-value as indicated) followed by post hoc Student's t tests. *Significantly different from the isotype group. n = 9-11 per group. b, Micrographs of different subsets of leukocytes stained for Ly6G or CD3 by immunohistochemistry in brain sections from mice killed 13 days after EAE induction. Scale bar = 5 μm. c, Counts of leukocytes in the cerebral cortex of EAE mice treated or not with anti-CXCL1 antibody and killed at day 13. The means were compared using the Student's t test. *Significantly different from the isotype group. n = 9-11 per group. d, Micrographs of granulocytes located inside (arrows) or outside (arrowheads) brain capillaries in a EAE mouse. Black = Ly6G immunoperoxidase staining using nickel-DAB as a substrate. Red-brown = CD31 immunoperoxidase staining using DAB as a substrate. Scale bar = 10 μm.Back to article page