Contribution of CXCL1 to the development of EAE. a, Clinical scores of EAE mice injected intravenously once daily from day 7 with 4 mg/kg anti-CXCL1 antibody or isotypic control antibody. The means were compared by multivariate ANOVA with repeated measures (P-value as indicated) followed by post hoc Student's t tests. *Significantly different from the isotype group. n = 9-11 per group. b, Micrographs of different subsets of leukocytes stained for Ly6G or CD3 by immunohistochemistry in brain sections from mice killed 13 days after EAE induction. Scale bar = 5 μm. c, Counts of leukocytes in the cerebral cortex of EAE mice treated or not with anti-CXCL1 antibody and killed at day 13. The means were compared using the Student's t test. *Significantly different from the isotype group. n = 9-11 per group. d, Micrographs of granulocytes located inside (arrows) or outside (arrowheads) brain capillaries in a EAE mouse. Black = Ly6G immunoperoxidase staining using nickel-DAB as a substrate. Red-brown = CD31 immunoperoxidase staining using DAB as a substrate. Scale bar = 10 μm.