Pre-treatment of glycyrrhizin (GL) once daily for 4 days reduces tibial nerve injury (TNI) induced pain hypersensitivity. The glycyrrhizin treatment paradigm includes a one-time injection of glycyrrhizin (i.p.; 50 mg/kg) or treatment with glycyrrhizin one injection per day over four consecutive days (A). (B) A one-time injection of glycyrrhizin at TNI [PID] 7 or [PID] 56 produced only a partial effect on the paw withdrawal threshold (PWT). Treatment with glycyrrhizin injections over four consecutive days [PID11-14] or [PID61-64] successfully reversed TNI decreases in PWT (*p < 0.01).