Figure 1From: Recurrent/moderate hypoglycemia induces hippocampal dendritic injury, microglial activation, and cognitive impairment in diabetic rats Neuronal death in the cerebral cortex and hippocampus after severe or moderate hypoglycemia in diabetic rats. (A) In sham hypoglycemia-operated rats, neuronal death (FJB (+) neurons) was not detected in diabetic rats. In both diabetic and non-diabetic rats, recurrent/moderate (R/M) hypoglycemia shows only occasional neuronal death in the cerebral cortex while acute/severe (A/S) hypoglycemia shows widespread neuronal death in the superficial layer of cortex at 7 days after hypoglycemia. Bar = 100 μm. (B) Bar graph represents counted degenerating neurons in the cerebral cortex. Data are mean + s.e.m.; n = 5 in R/M hypoglycemia, n = 7 in A/S hypoglycemia * P < 0.05. (C) R/M hypoglycemia shows no neuronal death (FJB (+) neurons) in the hippocampal CA1 and DG cell layer while severe hypoglycemia shows substantial neuron death in the hippocampus at seven days after hypoglycemia. Bar = 100 μm. (D) Light microscopic images represent hematoxylin & eosin (H&E) stained hippocampus from CA1 and DG cell layer. R/M hypoglycemia shows no neuronal death (eosinophilic) in the hippocampal CA1 and DG cell layer while severe hypoglycemia shows substantial neuronal death in the hippocampus at seven days after hypoglycemia. Bar = 100 μm.Back to article page