R/M hypoglycemia decreases dendrite density and thickness of stratum radiatum (SR) and stratum moleculare (SM) layer of hippocampal CA1 8 weeks after injury. (A) Fluorescent images show MAP2 (green) antibody stained stratum radiatum (SR) stratum moleculare (SM) of hippocampal CA1 area 8 weeks after R/M hypoglycemia. The MAP2 intensity in the dendritic area after R/M hypoglycemia (R/M HG) is substantially decreased. This MAP2 intensity decrease is more obvious in the diabetic rats, which suggests R/M hypoglycemia-induced dendrite loss in the SR and SM of hippocampus is worse in the diabetic animal. SP, Stratum Pyramidalae. Bar = 200 μm. (B) Bar graph represents quantitated MAP2 fluorescent intensity from the SR area of hippocampal CA1 area. Square in (A) shows evaluated area of MAP2 fluorescent intensity. (C) Bar graph represents thickness of SR + SM area of hippocampal CA1 area. Data are mean + s.e.m.; n = 7, * P < 0.05.