Representative gels (inset) or temporal changes in Bcl-2, Bax or cytochrome
relative to β-actin protein detected in the cytosolic fraction or relative to cytochrome
oxidase subunit IV (COX-IV) in the mitochondrial fraction of samples collected from the CA3 subfield of the hippocampus after microinjection of kainic acid (KA) or PBS. (A) Cytosolic fraction and (B) mitochondrial fraction of samples collected 3, 6, 12, 24 and 48 h after microinjection of KA (0.5 nmol) or PBS into the left hippocampal CA3 subfield. β-actin or COX-IV was used as the internal loading control for the cytosolic or mitochondrial fraction. Values are mean ± standard error of the mean (SEM) of the ratio of Bcl-2, Bax or cytochrome c to the loading controls, and are quadruplicate analyses from six animals per experimental group. *P < 0.05 versus sham-control (S) group in the Scheffé multiple-range test.