Temporal changes in peroxisome proliferator-activated receptor γ (PPARγ) protein relative to β-actin protein and UCP2 protein relative to cytochrome
oxidase subunit IV (COX-IV), and real-time PCR analysis showing fold changes in uncoupling protein 2 (
) mRNA expression relative to glyceraldehyde-3-phosphate dehydrogenase after microinjection of kainic acid or PBS into the left hippocampal CA3 subfield. (A) Changes in PPARγ protein relative to β-actin protein. (B) Fold changes in Ucp2 mRNA expression relative to glyceraldehyde-3-phosphate dehydrogenase. (C) Changes in UCP2 protein relative to COX-IV. Changes were detected in samples collected from the CA3 subfield of the hippocampus at 3, 6, 12, 24 or 48 h after microinjection of 0.5 nmol kainic acid or PBS into the left hippocampal CA3 subfield. Values are mean ± standard error of the mean (SEM) of quadruplicate analyses from six animals per experimental group. *P < 0.05 versus sham-control group (S) in the Scheffé multiple-range test.