Microglial podosomes are enriched in phosphotyrosine and the Src substrate Tks5. Deconvolved, color-separated and merged images of entire podonuts are shown in the upper panels, and the boxed areas are shown magnified and color-separated below. Scale bar: 5 μm (upper), 2 μm (lower). (A) Immunostaining for phosphotyrosine residues (pTyr, red) is enriched in the F-actin-rich podonut (labeled with phalloidin, green). At higher magnification, some co-localization is seen. (B) Immunostaining shows tyrosine kinase substrate with five Src homology 3 domains (Tks5; green) in microglia podosomes together with the ring marker talin (red). The small punctae of Tks5 are often adjacent to the talin staining.