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Table 1 Antibodies and stains used

From: Podosomes in migrating microglia: components and matrix degradation

Antibody/stain (species, type) Concentration Source
Phalloidin 488 or 350 (labels F-actin) 1:50 Invitrogen (Carlsbad, CA, USA)
DAPI (4′,6-Diamidino-2-phenylindole; labels DNA) 1:3,000 Invitrogen
Talin1/2 (mouse monoclonal) 1:100 Abcam (Cambridge, MA, USA)
Arp2 (actin-related protein 2) (rabbit polyclonal) 1:100 Santa Cruz Biotechnology (Santa Cruz, CA, USA)
Tks5 (tyrosine kinase substrate with five Src homology 3 domains) (rabbit polyclonal) 1:100 Santa Cruz Biotechnology
Phospho-caveolin 1 (p-Tyr14Cav1) 1:100 Signalway Antibody
(rabbit polyclonal) (Pearland, TX, USA)
Anti-phosphotyrosine (mouse monoclonal) 1:100 Abcam
Nox1 (nicotinamide adenine dinucleotide phosphate oxidase 1) (goat polyclonal) 1:100 Santa Cruz Biotechnology
DyLight donkey anti-rabbit 488 or 594 (secondary) 1:250 Jackson Immunoresearch (West Grove, PA, USA)
DyLight donkey anti-mouse 488 or 594 (secondary) 1:250 Jackson Immunoresearch
Donkey anti-goat FITC (secondary) 1:250 Jackson Immunoresearch