Table 1 Antibodies and stains used
From: Podosomes in migrating microglia: components and matrix degradation
Antibody/stain (species, type) | Concentration | Source |
|---|---|---|
Phalloidin 488 or 350 (labels F-actin) | 1:50 | Invitrogen (Carlsbad, CA, USA) |
DAPI (4′,6-Diamidino-2-phenylindole; labels DNA) | 1:3,000 | Invitrogen |
Talin1/2 (mouse monoclonal) | 1:100 | Abcam (Cambridge, MA, USA) |
Arp2 (actin-related protein 2) (rabbit polyclonal) | 1:100 | Santa Cruz Biotechnology (Santa Cruz, CA, USA) |
Tks5 (tyrosine kinase substrate with five Src homology 3 domains) (rabbit polyclonal) | 1:100 | Santa Cruz Biotechnology |
Phospho-caveolin 1 (p-Tyr14Cav1) | 1:100 | Signalway Antibody |
(rabbit polyclonal) | (Pearland, TX, USA) | |
Anti-phosphotyrosine (mouse monoclonal) | 1:100 | Abcam |
Nox1 (nicotinamide adenine dinucleotide phosphate oxidase 1) (goat polyclonal) | 1:100 | Santa Cruz Biotechnology |
DyLight donkey anti-rabbit 488 or 594 (secondary) | 1:250 | Jackson Immunoresearch (West Grove, PA, USA) |
DyLight donkey anti-mouse 488 or 594 (secondary) | 1:250 | Jackson Immunoresearch |
Donkey anti-goat FITC (secondary) | 1:250 | Jackson Immunoresearch |