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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Toll-like receptor 2 deficiency leads to delayed exacerbation of ischemic injury

Figure 2

Toll-like receptor 2 deficiency leads to altered microglia/macrophage activation profiles. (A) Schematic representation of the brain section used for the immunohistochemical experiments. Box represents the area magnified in B and D, and in Figure 3A,C,F,G, as well as in Figures 6 and 7. (B) Representative photomicrographs of Iba1 immunoreactivity in control (unlesioned) wild-type (WT) and Toll-like receptor (TLR) 2−/− mice, as well as 3 and 7 days after transient middle cerebral artery occlusion (MCAO), indicate reduced accumulation of Iba1-positive cells in TLR2 deficient mice. (C) Quantification of Iba1 immunoreactivity revealed significantly decreased immunoreactivity in TLR2−/− mice compared with the WT group of mice 3 and 7 days after transient MCAO. (D) Photomicrographs of CD68 immunoreactivity in unlesioned (control) WT and TLR2−/− mice 3 and 7 days after transient ischemic lesion. (E) Quantitative analysis of CD68 immunoreactivity in both groups reveals significant reduction of the signal intensity 7 days after MCAO in the TLR2−/− group. Values (C, E) indicate mean ± SEM (n = 4; **P <0.001 ***P <0.0001). Scale bars: B, D: 100 μm.

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