Figure 2

ApoE genotype influences apoC-I expression in humanized mice. Liver (A), serum (B), and cerebral cortex (C) were collected from 12 week-old targeted replacement APOE mice homozygous for ϵ3 or ϵ4. Total RNA was isolated from liver (A) and cortex (C) and qPCR performed to quantify apoC-I mRNA expression from each genotype. Serum (B) was assayed for apoC-I expression using ELISA. (D) Total RNA was isolated from primary astrocyte cultures prepared from humanized APOE mice, and qPCR performed to quantify apoC-I mRNA expression from each genotype. Expression of apoC-I from all tissues analyzed was significantly lower in ϵ4 animals compared to ϵ3. Data are expressed as the mean ± standard error of the mean (SEM) percentage apoC-I mRNA (A, C, D) of ϵ3 mice or protein concentration (B); n = 4 to 6. *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test.