Figure 3

ApoC-I suppresses toll-like receptor (TLR)-dependent activation of primary microglia. Cultured WT primary murine microglia were treated with 1 μg/ml Pam3 (TLR2), 20 μg/ml PIC (TLR3), 100 ng/ml LPS (TLR4), or 1 μM CpG (TLR9) for 18 h in the presence or absence of 1 μM apoC-I and supernatant amounts of (A) IL-6 and (B) TNF-α (pg/μg protein) quantified by ELISA. ApoC-I significantly reduced PIC and LPS stimulated cytokine release from microglia, but had no effect on cells treated with Pam3 or CpG. Unstimulated vehicle controls were below the level of detection (data not shown). Data are expressed as the mean ± standard error of the mean (SEM) cytokine amount; n = 3 to 6. ***P < 0.001; analysis of variance (ANOVA) with Bonferroni’s multiple comparison test.