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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Apolipoprotein C-I is an APOE genotype-dependent suppressor of glial activation

Figure 4

ApoC-I suppression of toll-like receptor (TLR)-mediated microglial activation is dose and receptor-associated protein (RAP) dependent. (A) Cultured WT primary mouse microglia were treated with 20 μg/ml PIC and various concentrations of ApoC-I as indicated for 18 h and supernatant amounts of IL-6 and TNF-α (pg/μg protein) quantified by ELISA. Data are expressed as mean ± standard error of the mean (SEM) percentage of the maximum PIC stimulation for each analyte; n = 3 to 5. (B) RAP significantly reversed apoC-I suppression of PIC-stimulated secretion of IL-6 and TNF-α by microglia. WT microglia were treated with 20 μg/ml PIC for 18 h in the presence of apoC-I (1 μM) and/or RAP (1 μM) and cytokine secretion measured by ELISA. Unstimulated vehicle controls were below the level of detection, and treatment with RAP alone did not stimulate cytokine release (data not shown). Data are expressed as mean ± SEM percentage of maximum PIC stimulation; n = 3 to 5. ***P < 0.001, analysis of variance (ANOVA) with Bonferroni’s multiple comparison test.

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