Figure 6

Toll-like receptor (TLR)-dependent cytokine expression in human macrophages is suppressed by apoC-I. Differentiated THP-1 cells were treated with 20 μg/ml PIC or 100 ng/ml LPS for 8 h in the presence or absence of 1 μM apoC-I and total RNA isolated from cells. qPCR analysis showed a significant stimulation of (A) IL-6 and (B) TNF-α mRNAs with both PIC and LPS treatment. Co-administration with apoC-I significantly reversed this effect. Data are expressed as mean ± standard error of the mean (SEM) fold induction; n = 3. *P < 0.05; **P < 0.01; ***P < 0.001, analysis of variance (ANOVA) with Bonferroni’s multiple comparison test.