Skip to main content

Table 1 Change in primary microglia response to 20 μg/ml PIC exposure in the presence or absence of 1 μM apoC-I

From: Apolipoprotein C-I is an APOE genotype-dependent suppressor of glial activation

 

Concentration (pg/ml)

Change (%)

 

Vehicle

PIC

PIC/apoC-I

Vehicle vs. PIC

PIC vs. PIC/apoC-I

G-CSF

ND

34.3

11.0

-

−68

IFN-γ

ND

12.8

9.3

-

−27

IL-1α

13.4

39.6

24.7

196

−38

IL-1β

ND

9.8

1.8

-

−82

IL-6

ND

795

28.9

-

−96

IL-13

ND

28.7

23.7

-

−17

IL-17

ND

13.2

8.6

-

−35

IP-10

ND

731.8

743.3

-

2

KC

ND

435.9

83.3

-

−81

LIX

83.6

193.7

99.9

132

−48

MCP-1

18.2

175.8

61

866

−65

M-CSF

4.3

9.4

6.8

119

−28

MIG

ND

604.6

599.1

-

−1

MIP-1α

41.2

2737.4

796.9

6544

−71

MIP-1β

ND

2128

1783.7

-

−16

MIP-2

38.2

3173.9

262.3

8209

−92

RANTES

ND

362

327.7

-

−9

TNF-α

7.4

409

124.4

5427

−70

  1. WT murine primary microglia were exposed to 20 μg/ml PIC or 100 ng/ml LPS for 18 h in the presence or absence of 1 μM apoC-I, and supernatants were assayed with a 32-plex array for Eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-15, IL-17, interferon gamma-induced protein 10 (IP-10), keratinocyte-derived chemokine (KC), leukemia inhibitory factor (LIF), lipopolysaccharide-induced CXC chemokine (LIX), monocyte chemotactic protein-1 (MCP-1), macrophage-colony stimulating factor (M-CSF), monokine induced by gamma interferon (MIG), macrophage inflammatory protein (MIP)-1α, MIP-1β, MIP-2, regulated upon activation normal T-cell expressed and secreted (RANTES), TNF-α, and vascular endothelial growth factor (VEGF). PIC exposure resulted in two- to eighty-fold increased concentrations of seven analytes with measurable baseline (vehicle control) levels, and increased concentration in eleven other analytes in which baseline levels were undetectable. Of these, seven showed two- to eighty-fold induction over baseline (vehicle-control) levels and 11 others were induced for which there was no detectable baseline protein by PIC. ApoC-I co-exposure effectively reduced 12 of these PIC-stimulated cytokines. Data are expressed as cytokine amounts (pg/μg protein) from two independent experiments pooled from 3 replicate cultures and run in duplicate. ND: not detectable; PIC: polyinosinic-polycytidylic acid.