| Concentration (pg/ml) | Change (%) |
---|
| Vehicle | PIC | PIC/apoC-I | Vehicle vs. PIC | PIC vs. PIC/apoC-I |
---|
G-CSF | ND | 34.3 | 11.0 | - | −68 |
IFN-γ | ND | 12.8 | 9.3 | - | −27 |
IL-1α | 13.4 | 39.6 | 24.7 | 196 | −38 |
IL-1β | ND | 9.8 | 1.8 | - | −82 |
IL-6 | ND | 795 | 28.9 | - | −96 |
IL-13 | ND | 28.7 | 23.7 | - | −17 |
IL-17 | ND | 13.2 | 8.6 | - | −35 |
IP-10 | ND | 731.8 | 743.3 | - | 2 |
KC | ND | 435.9 | 83.3 | - | −81 |
LIX | 83.6 | 193.7 | 99.9 | 132 | −48 |
MCP-1 | 18.2 | 175.8 | 61 | 866 | −65 |
M-CSF | 4.3 | 9.4 | 6.8 | 119 | −28 |
MIG | ND | 604.6 | 599.1 | - | −1 |
MIP-1α | 41.2 | 2737.4 | 796.9 | 6544 | −71 |
MIP-1β | ND | 2128 | 1783.7 | - | −16 |
MIP-2 | 38.2 | 3173.9 | 262.3 | 8209 | −92 |
RANTES | ND | 362 | 327.7 | - | −9 |
TNF-α | 7.4 | 409 | 124.4 | 5427 | −70 |
- WT murine primary microglia were exposed to 20 μg/ml PIC or 100 ng/ml LPS for 18 h in the presence or absence of 1 μM apoC-I, and supernatants were assayed with a 32-plex array for Eotaxin, granulocyte-colony stimulating factor (G-CSF), granulocyte macrophage-colony stimulating factor (GM-CSF), IFNγ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12(p40), IL-12(p70), IL-13, IL-15, IL-17, interferon gamma-induced protein 10 (IP-10), keratinocyte-derived chemokine (KC), leukemia inhibitory factor (LIF), lipopolysaccharide-induced CXC chemokine (LIX), monocyte chemotactic protein-1 (MCP-1), macrophage-colony stimulating factor (M-CSF), monokine induced by gamma interferon (MIG), macrophage inflammatory protein (MIP)-1α, MIP-1β, MIP-2, regulated upon activation normal T-cell expressed and secreted (RANTES), TNF-α, and vascular endothelial growth factor (VEGF). PIC exposure resulted in two- to eighty-fold increased concentrations of seven analytes with measurable baseline (vehicle control) levels, and increased concentration in eleven other analytes in which baseline levels were undetectable. Of these, seven showed two- to eighty-fold induction over baseline (vehicle-control) levels and 11 others were induced for which there was no detectable baseline protein by PIC. ApoC-I co-exposure effectively reduced 12 of these PIC-stimulated cytokines. Data are expressed as cytokine amounts (pg/μg protein) from two independent experiments pooled from 3 replicate cultures and run in duplicate. ND: not detectable; PIC: polyinosinic-polycytidylic acid.