Chemokine, chemokine receptor, and adhesion molecule genetic expression in the brains of wild type and CD73−/− mice following EAE induction. (A) EAE disease profile of wild type (n = 8) and CD73−/− mice (n = 10). Error bars represent the s.e.m. Significant differences are indicated as determined by two-way ANOVA. EAE data is combined from 2 separate experiments. (B) CD45 and CD4 stained frozen brain sections (cerebellum) from day 14 post-EAE induction wild type and CD73−/− mice. Positively stained cells (red) are shown against a hematoxylin counterstain (blue). Black scale bars represent 50 μm. (C) Brain gene expression from wild type and CD73−/− mice with EAE over time (n = 5 mice per group) as determined by quantitative real-time PCR. Gene levels were normalized to GAPDH levels and displayed in a heat map as a ratio of wild type to CD73−/− to determine fold differences in expression at each time point. (D) Brain gene expression from wild type mice treated with the broad spectrum adenosine receptor agonist NECA (0.25 mg/kg) or DMSO vehicle control as determined by quantitative real-time PCR 4 hours post treatment. Gene levels were normalized to GAPDH levels and displayed in a heat map as a ratio of NECA to vehicle treated to determine fold differences in expression.