Astrocyte elevated gene-1 (AEG-1) localizes to the astrocyte nucleolus during wound healing. At 48 h post injury astrocytes were immunostained for fibrillarin (red, nucleolar marker, (A)), AEG-1 (green, (B)) and 4', 6-diamidino-2-phenylindole (DAPI) (blue, nuclear marker, (C)) and micrographed by confocal microscopy. Z-stack micrographs of fibrillarin fluorescence were overlaid upon AEG-1 and DAPI (D). Higher magnification micrograph of AEG-1 and fibrillarin costaining is shown (E). A scatter plot of overlapping AEG-1 and fibrillarin pixels was generated (P = 1, (F)). Representative confocal images are 200 × original magnification from 2 individual donors assayed in triplicate.