IL-1β treated differentiated SH-SY5Y cells. The cells were treated with IL-1β (50, 100, or 150 ng/ mL) for described times. (A) BACE-1 (70 kD) increased particularly in 48-hr IL-1β treatment (50 ng/mL) compared to the untreated control. Actin was used as loading control. (B) IL-1β increased protein level of NTF of PS-1 (34 kD), whereas protein synthesis of precursor PS-1 (53 kD) decreased in 6-h or 24-h IL-1β treated cells compared to untreated control. (C) IL-1β treatment increased protein levels of APP concentration dependently in 6-h treated cells. (D) IL-1β treatment increased slightly Aβ production. (E) Bcl-xL reduced in 6-h IL-1β treated cells but increased in the final 72-h time point. Ctrl, untreated control; NTF, N-terminal fragment of PS-1.