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Figure 3 | Journal of Neuroinflammation

Figure 3

From: Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia

Figure 3

Effect of rapamycin, a mTOR inhibitor, and NVP-BEZ235, a dual PI3K/mTOR inhibitor, on COX-2 and mPGES-1 immunoreactivity and PGE 2 and PGD 2 release in primary LPS-stimulated rat microglia. A: Immunoblot analysis of protein levels of COX-2, mPGES-1 and β-actin in LPS-activated microglia treated with rapamycin (0.05-2.5 nM). B: Quantitative densitometric analysis of COX-2 and mPGES-1 protein expression normalized to β-actin loading control. C: Effect of rapamycin (0.05-2.5 nM) on PGE2 and PGD2 production after 48 h of LPS stimulation in rat primary microglia. D: Effect of incubation of rat microglia with rapamycin (0.05-2.5 nM) on PGE2 and PGD2 release in absence of LPS. *P < 0.05 with respect to LPS control.

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