Figure 2From: IgG glycan hydrolysis by EndoS inhibits experimental autoimmune encephalomyelitis Endoglycosidase treatment has a profound effect on IgG glycan hydrolysis in vivo . (A) Total serum IgG levels isolated from EndoS-treated WT mice (n = 6) and PBS-treated treated WT mice (n = 4) at the indicated time after immunization were determined by ELISA. (B) SDS-PAGE Coomassie Blue staining (stain) and lectin blot analysis (LCA) of purified IgG from serum samples drawn from three representative PBS- or EndoS-treated WT mice at indicated times after intravenous injection of 20 μg EndoS or PBS (1 and 8 days).Back to article page