Figure 4

Ectopic expression of microRNA (miR)-181c attenuates oxygen-glucose deprivation-activated BV2-induced neuronal apoptosis. (A-B) BV-2 cells were transfected with miR-181c mimics or NC 24 hoursbefore activation by OGD. After 48 hours, the production levels of TNF-α (A) and NO (B, top) were determined by ELISA and the Griess assay, respectively. The cells were harvested at 48 hours after OGD, and iNOS protein expression was evaluated by western blotting (B, lower). (C-D) BV-2 cells were transfected with miR-181c mimics or NC 24 hoursbefore activation by OGD. After 48 hours, the conditioned media were harvested. Cultured neurons were grown in the microglia-conditioned medium (MCM) and then analyzed for apoptosis using Hoechst 33342 staining. The percentage of apoptotic cells in the total neuronal population was calculated (C). Representative photographs are shown in (D). The results are presented as the mean ± SE from three independent experiments. NC represents thenegative control for the miR-181c mimics. *P < 0.05.