Intraperitoneal infusion of LPS induces endothelial dysfunction in RVLM. Representative gels (inset) and densitometric analysis of results from Western blot showing changes in expression of eNOS (A), iNOS (B), nNOS (C), or ICAM-1 (D), as well as photomicrographs showing distribution of vWF- (red fluorescence) and ICAM-1-immunoreactivity (green fluorescence) in RVLM, examined on day 7 after intraperitoneal infusion of saline or LPS (1.2 mg/kg/day), alone or with additional intracisternal infusion of NS398 (1.5 nmol/μL/h). Values are mean ± SEM (n=8 to 10 animals in each experimental group). *P <0.05 vs. saline-treatment group; #
P <0.05 vs. LPS-treatment group in the post hoc Scheffé multiple-range test. Note that colocalization of vWF- and ICAM-1-immunoreactivity is shown in yellow color in (D). Scale bar in (D): 10 μm.