Expression of the NAD(P)H oxidase subunits Cybb/NOX2 (gp91
) and Ncf1 (p47
) in the optic nerve of mice with ON. (A) Gene expression was assessed in naïve animals and at 8 and 11 dpi. For each gene, results were expressed as fold of corresponding WT naive ± SEM after normalization to β-actin. Four mice/group/time were analyzed. *P < 0.05, one-way ANOVA, Tukey test. (B) p47phox immunohistochemistry in the optic nerve of WT and GFAP-IκBα-dn mice in naïve conditions and at 11 and 20 dpi. Arrows point at astrocytes colocalizing with p47phox protein. Yellow arrows identify the cells magnified in the inserts. Scale bar = 20 μm.