Time-course of RGC loss in mice with ON. (A) Immunohistochemistry with the pan-neuronal marker NeuN in flat-mounted retinas of WT and GFAP-IκBα-dn mice under naïve conditions and at various times after induction of EAE. (B) Quantification of NeuN-labeled RGCs with MetaMorph software. *P < 0.05, one-way ANOVA, Tukey test. Ten mice/group were analyzed. Scale bar = 50 μm.