Table 1 Primers for real-time PCR amplification
Gene | Oligonucleotides | PCR product size | |
|---|---|---|---|
IL-1β | Forward | GACCTTCCAGGATGAGGACA | 283 bp |
Reverse | AGGCCACAGGTATTTTGTCG | ||
CCL5 | Forward | AGCAGCAAGTGCTCCAATCT | 280 bp |
Reverse | ATTTCTTGGGTTTGCTGTGC | ||
CXCL10 | Forward | GCTGCAACTGCATCCATATC | 293 bp |
Reverse | CACTGGGTAAAGGGGAGTGA | ||
ICAM-1 | Forward | TGGTGATGCTCAGGTATCCA | 273 bp |
Reverse | CACACTCTCCGGAAACGAAT | ||
TNF | Forward | CAAAATTCGAGTGACAAGCCTG | 113 bp |
Reverse | GAGATCCATGCCGTTGGC | ||
NOS2 | Forward | CAGAGGACCCAGAGACAAGC | 299 bp |
Reverse | TGCTGAAACATTTCCTGTGC | ||
Cybb/NOX2 | Forward | GACTGCGGAGAGTTTGGAAG | 258 bp |
Reverse | ACTGTCCCACCTCCATCTTG | ||
Ncf1 | Forward | CGAGAAGAGTTCGGGAACAG | 286 bp |
Reverse | AGCCATCCAGGAGCTTATGA | ||
β-actin | Forward | CACCCTGTGCTGCTCACC | 327 bp |
Reverse | GCACGATTTCCCTCTCAG | ||