Specificity of B1R antibody for immunolocalization. Shown are confocal microscopy pictures of coronal sections of subdiaphragmatic vagus nerve isolated from STZ rats labeled with pre-immune anti-B1R (A, green) and anti-CGRP (B, red). Picture overlay is presented in panel C showing no evidence of co-localization (no yellow color). Images are representative of at least four sections from three rats. Scale bar = 100 μm.