Figure 2
From: Complement is dispensable for neurodegeneration in Niemann-Pick disease type C
C1qa expression is induced in microglia in the brains of NPC mice. (A) In wild-type mice, C1qa mRNA (left panel) and C1q protein (right panel) localize to cerebellar PNs (yellow arrows; anti-D28K green). A rendering of stacked confocal images (right panel) shows speckles of C1q (white arrow) internal to and proximal to the neuron. (B) In Npc1 -/- mice, C1qa mRNA (left panel) and C1q protein (right panel) do not localize entirely to PNs. (C) Overlay (right panel) of C1qa mRNA (left panel, magenta) with anti-D28K (middle panel, green). (D, top panel) NBT/BCIP reaction from C1qa RNA in situ hybridization (dark purple) co-localizes with DAB anti-CD68 immunohistochemistry (brown) in Npc1 -/- mice. (D, bottom panel) In C1qa -/-; Npc1 -/- mice, only the DAB staining can be seen. Scale bars are 10μm.