Levels of virus-specific CD8
T cell responses in the CNS of virus-infected B6 and IL-1R KO mice. (A) Levels of H-2Db-VP2-121–130-tetramer reactive CD8+ T cells in the central nervous system (CNS) of B6 and IL-1R knockout (KO) mice at 8 days post-infection (dpi). (B) Proportions of CNS-infiltrating CD8+ T cells reactive to viral epitopes, anti-CD3/CD28 antibodies and PMA/ionomycin were assessed using flow cytometry following intracellular cytokine staining at 8 dpi. (C) The overall numbers of virus-specific and anti-CD3/CD28 reactive CD8+ T cells in the CNS of virus-infected B6 and IL-1R KO mice are shown at 8 and 21 dpi. A representative result from two to three similar experiments is shown here.