Figure 8

QD-saporin-mediated depletion of microglia decreases neuronal loss in mixed cortical cultures exposed to Aβ. A. Representative photomicrographs of microglial cells in mixed cortical cultures treated with QDs or QD-saporin (QD-Sap) conjugates. Microglial cells were labeled with anti-Iba-1 (green) and nuclei were labeled with DAPI (blue). B. Quantification of Iba-1-positive cells in mixed cultures treated with unconjugated saporin (Sap), QDs, or QD-saporin conjugates (QD-Sap). C. Quantification of MAP2-positive neurons in mixed cultures treated with Aβ1-42 after pretreatment with medium alone, QDs, or QD-Sap. n = 5 wells from three independent experiments. *P < 0.05, **P < 0.01, one-way ANOVA with Tukey-Kramer posthoc analyses. Scale bar, 100 μm.