Neuronal and synaptic degeneration in AAV2-P301L tau-injected hemisphere. Microglial marker (A,B, CD68), neuronal (C,D, NeuN), dendritic (E,F, MAP-2), and synaptic (G,H, synaptophysin) integrity was quantified based on mean % area of immunoreactivity (IR, for example, neuropil occupied for the respective markers) (B,F,H) or IR alone (D). MAP-2 was quantified lateral to injection site to avoid overlap with the GFP signal. Each symbol indicates the mean number of 3 to 4 sections per mouse. Significance was calculated by ANOVA and post-hoc Tukey-Kramer test; *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001. Scale bar, 100 μm (A), 200 μm (B), and 50 μm (D,E).