Localization of fluorescently-tagged small interfering (si)RNA following intravitreal injection in control retinas.
(A) Fluorescence was not detected in retinas injected with Invivofectamine only in mice reared in dim light. (B) Strong fluorescence for injected siRNA (red) was seen after 24 hours of incubation, which was apparent throughout all cellular layers through to the outer nuclear layer (ONL). (C-I) Dual immunolabeling of siRNA (red) and the Müller-cell-specific proteins S100β/vimentin (green) showed colocalization of siRNA with S100β/vimentin in Müller cell processes situated within the (C–F) ONL and outer limiting membrane (OLM) (arrows), and (G–I) the inner nuclear layer (INL) (arrows). GCL, ganglion cell layer; IPL, inner plexiform layer; OS, outer segments.