Figure 3

Chemokine (C-C motif) ligand (Ccl)2 expression in Müller cells following Ccl2 small interfering (si)RNA treatment and light damage. (A–C) Representative images taken from the superior mid-periphery showing in situ hybridization results for Ccl2 mRNA within processes situated in the inner nuclear layer (INL) following exposure to bright continuous light (BCL; black arrows). (D) Sense controls showed no specific staining. (E–G) Co-labelling for Ccl2 mRNA with a fluorescent stain (red) showed colocalization for vimentin-immunoreactive (green) Müller cell processes (white arrows). The histogram shows that the number of Ccl2-expressing Müller cells per retina decreased significantly in the Ccl2 siRNA-treated group (12.6 cells) compared with the Invivofectamine-only (47.4 cells; P < 0.05) and the scrambled siRNA (50.1 cells; P < 0.05) groups. Inivofectamine-only (n = 4), scrambled siRNA (n = 4), Ccl2 siRNA (n = 4). Error bars represent SEM. *Significant change at P <0.05 using ANOVA with Tukey’s post hoc test. ONL, outer nuclear layer.