Figure 4

Immunoreactivity (IR) for chemokine (C-C motif) ligand (Ccl)2 protein in Müller cells following light damage in relation to Ccl2 small interfering (si)RNA treatment. (A–D) Representative images from the superior mid-periphery showed strong IR for Ccl2 (red) in radially oriented processes within the inner nuclear layer (INL) following (A–C) BCL (white arrows), whereas (D) negative controls showed no fluorescence. (E–G) The Ccl2 IR showed strong colocalization for S100B-immunoreactive (green) Müller cell processes (white arrows). The histogram of the quantification of Ccl2-IR Müller cell processes per retina indicated a substantial reduction in animals injected with Ccl2 siRNA (11.6, P < 0.05) compared with those injected with Invivofectamine only or with scrambled siRNAs (45.1 and 45.2 respectively). Inivofectamine-only (n = 4), scrambled siRNA (n = 4), Ccl2 siRNA (n = 4) Error bars represent SEM. *Significant change at P <0.05 using ANOVA with Tukey’s post hoc test. ONL, outer nuclear layer.