Recruitment of monocytes positive for ED1 and ionized calcium binding adaptor molecule (IBA)1 following treatment with bright continuous light (BCL) in retinas injected with chemokine (C-C motif) ligand (Ccl)2 small interfering (si)RNA. (A-F) Representative images taken from the superior mid-periphery showed immunoreactivity for ED1 (green) and IBA1 (red) in ED1+/IBA1+ nuclei recruited to the retinal vasculature following BCL (arrows). (G) The total number of these ED1+/IBA1+ nuceli per retina was significantly reduced in Ccl2 siRNA-injected retinas following BCL compared with the Invivofectamine-only group and the scrambled siRNA control group (P < 0.05). (H) Numbers of ED1+/IBA1+ nuclei recruited to retinal and choroidal vasculature locations were reduced in retinas injected with Ccl2 siRNA in comparison to controls (P < 0.05). Inivofectamine-only (n = 4), scrambled siRNA (n = 4), Ccl2 siRNA (n = 4) Error bars represent SEM. *Significant change at P <0.05 using ANOVA with Tukey’s post hoc test. GCL, ganglion cell layer; IPL, inner plexiform layer.