Figure 6From: Small interfering RNA-mediated suppression of Ccl2 in Müller cells attenuates microglial recruitment and photoreceptor death following retinal degeneration Recruitment of microglia positive for ionized calcium binding adaptor molecule (IBA)1 and negative for ED1 following BCL in retinas injected with chemokine (C-C motif) ligand (Ccl)2 small interfering (si)RNA. (A–F) Representative images from the superior mid-periphery showed immunoreactivity for IBA1 (red), but not ED1 (green), in ramified ED1−/IBA1+ nuclei that were recruited to the outer nuclear layer (ONL) and outer plexiform layer (OPL) after 24 hours of BCL (A–C; arrows). (G) The total number of these recruited ED1−/IBA1+ nuclei per retina decreased significantly in Ccl2 siRNA-injected retinas following BCL, compared with both the Invivofectamine-only group and the scrambled siRNA control group (P < 0.05). (H) Numbers of ED1−/IBA1+ nuclei recruited to either the OPL or the ONL/subretinal space were reduced in retinas injected with Ccl2 siRNA in comparison with control groups (P < 0.05). Inivofectamine-only (n = 4), scrambled siRNA (n = 4), Ccl2 siRNA (n = 4). Error bars represent SEM. *Significant change at P <0.05 using ANOVA with Tukey’s post hoc test. INL, inner nuclear layer; OS, outer segments.Back to article page