Figure 4

The morphology of astrocytes was altered in the frontal cortex of NL3 knockdown mice. Immunohistochemistry studies were carried out on the whole brain sections from one NL3 knockdown mouse and one age-matched control littermate using an anti-glial fibrillary acidic protein (GFAP) antibody (dilution 1:1000). Immunostaining of GFAP (dark brown color) was present in all astrocytes. The density of astrocytes from the frontal cortex, as well as the number of astrocytic branch processes, cell body size and the total branch length were quantified using Image J analysis. *P < 0.05. Scale bar: 10 μm. Data are shown as mean ± standard error (SE).